Fig. 4

Attachment and uptake of PD-SK and PD-H to Colo320 cells. Colo320 cells were and infected with 0.1 MOI of PD-SK and PD-H. (A) Virus attachment. After infection the cells on ice, virus was removed, and cells were washed two times with ice-cold PBS and viral RNA isolated. Virus genome copy number was determined by quantitative RT-PCR. Shown are mean values ± SEM, Significance, * p < 0.05. (B) Virus uptake. After infection the cells on ice with PD-H and PD-SK Colo320 cells were incubated at 37 °C for 1 h. Cells were washed two times with ice-cold PBS and virus RNA isolated. Virus genome copy number was determined by quantitative RT-PCR. Shown are mean values ± SEM. Significance, * p < 0.05; ** p < 0.01